Related Experiment Video

Updated: Jan 22, 2026

Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay
09:21

Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay

Published on: June 17, 2010

50.5K

Ultrasensitive detection of miRNA via one-step rolling circle-quantitative PCR (RC-qPCR)

Mingcheng Xu1, Jiawei Ye1, Dan Yang1

  • 1Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, Zhejiang, 310058, China.

Analytica Chimica Acta
|July 17, 2019
PubMed

Keywords:
MiR-200aMiRNA detectionOne-step amplificationRolling circle amplificationRolling circle-quantitative PCR (RC-qPCR)Vent (exo-) DNA polymerase

More Related Videos

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Published on: December 3, 2010

17.8K
Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR10:55

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

Published on: November 10, 2018

27.9K

Related Concept Videos

Rolling Without Slipping01:09

Rolling Without Slipping

People have observed the rolling motion without slipping ever since the invention of the wheel. For example, one can look at the interaction between a car's tires and the surface of the road. If the driver presses the accelerator to the floor so that the tires spin without the car moving forward, there must be kinetic friction between the wheels and the road's surface. If the driver slowly presses the accelerator, causing the car to move forward, the tires roll without slipping. It is...
5.2K
Rolling With Slipping01:14

Rolling With Slipping

Rolling with slipping is a physical phenomenon that occurs when a rolling object experiences both rotational and linear motion but also experiences frictional forces that cause slipping. This phenomenon can occur in various situations, such as when a tire rolls on a wet road or a ball rolls on a rough surface.
An object's rolling motion is characterized by its rotation around its axis, while linear motion refers to the object's translational motion along a surface. Frictional forces can...
7.9K
RC Circuit with Source01:15

RC Circuit with Source

When a DC source is abruptly applied to an RC (Resistor-Capacitor) circuit, the voltage can be represented as a unit step function. The voltage across the capacitor, known as the step response, characterizes how the circuit reacts to this sudden change in input.
Due to the inherent properties of a capacitor, its voltage cannot change instantaneously. This means that immediately after the switch is closed, the capacitor's voltage remains the same as it was just before the switch was closed.
2.6K
PCR01:32

PCR

Overview
237.1K
RC Circuit without Source01:16

RC Circuit without Source

When a DC source is abruptly disconnected from an RC (Resistor-Capacitor) circuit, the circuit becomes source-free. Assuming that the capacitor was fully charged before the source was removed, its initial voltage, denoted as V0, can be considered as the initial energy that stimulates the circuit.
Applying Kirchhoff's current law at the top node of the circuit and substituting the current values across the components, a first-order differential equation is obtained. By rearranging the terms...
2.4K
Applications of RC Circuits01:22

Applications of RC Circuits

A relaxation oscillator is one of the applications of RC circuits. A neon lamp relaxation oscillator comprises a capacitor, a resistor, a voltage source, and a lamp. The lamp acts like an open circuit, with infinite resistance until the potential difference across the lamp reaches a specific voltage. At that voltage, the lamp acts like a short circuit with zero resistance, and the capacitor discharges through the lamp, thus producing light. Once the capacitor is fully discharged through the...
4.0K