Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.6K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The efficacy of immunotherapy in glioma requires distal B cell responses in tumor-draining lymph nodes.

Science immunology·2026
Same author

Urolithin A activates mitophagy via the AMPK-mTOR axis and modulates the gut-ceramide axis to ameliorate cardiac remodeling in HFpEF.

Experimental & molecular medicine·2026
Same author

Diagnostic accuracy of tachypnoea for predicting mortality and identifying sepsis in young infants aged 0-59 days: a systematic review and meta-analysis.

BMJ paediatrics open·2026
Same author

Enhancing Next-Generation Sequencing Sensitivity with High-Recovery Adapter Ligation and Cas9-Mediated Dimer Elimination.

Clinical chemistry·2026
Same author

Transcriptional Profiling at Single-Cell Resolution Reveals Diversity and Regulatory Networks of Primary and Secondary Senescent Cells.

Aging cell·2026
Same author

Long-term impact of health policy and management training for health experts from the LMICs: 2022 Dr. LEE Jong-wook fellowship program.

International health·2026

Related Experiment Video

Updated: Mar 11, 2026

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.6K

Dual-Gated G-Quadruplex DNAzyme-LAMP for Sequence-Specific and Positive Colorimetric Nucleic Acid Detection.

Hyun Shin1, Junhyeok Yoon1, Yumin Kim1

  • 1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.

ACS Sensors
|March 10, 2026
PubMed
Summary

This study presents a new colorimetric diagnostic test for Hepatitis A virus. The assay uses a dual-gated G-quadruplex DNAzyme-LAMP method for sensitive and specific detection, ideal for resource-limited settings.

Keywords:
DNAzymeG-quadruplexloop-mediated isothermal amplificationpathogen diagnosispositive colorimetric signalingsequence-specific signaling

More Related Videos

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.2K
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

2.0K

Related Experiment Videos

Last Updated: Mar 11, 2026

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.6K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.2K
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

2.0K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Diagnostics

Background:

  • Point-of-care nucleic acid diagnostics require rapid, instrument-free methods with high sensitivity and specificity.
  • Conventional colorimetric indicators in loop-mediated isothermal amplification (LAMP) can produce false positives due to non-specific DNA synthesis.

Purpose of the Study:

  • To develop a novel, sequence-specific colorimetric detection method for nucleic acid diagnostics.
  • To overcome the limitations of existing colorimetric LAMP assays, particularly false positives and reagent requirements.

Main Methods:

  • Developed a dual-gated G-quadruplex DNAzyme-LAMP assay integrating G-quadruplex DNAzyme formation into loop primers.
  • Utilized locked nucleic acid-stabilized probes to block non-specific activation.
  • Designed a system where target amplicons displace a blocking strand to gate colorimetric signal generation.

Main Results:

  • The assay demonstrated high sensitivity, detecting as few as 12 copies of Hepatitis A virus per reaction, comparable to RT-qPCR.
  • Achieved unambiguous positive colorimetric readouts with excellent specificity, even with a 10^9-fold excess of non-target DNA.
  • The platform utilizes inexpensive reagents (hemin, chromogenic substrates) and avoids complex logistics.

Conclusions:

  • The dual-gated G-quadruplex DNAzyme-LAMP platform offers a generalizable framework for reliable, sequence-specific colorimetric detection.
  • This method is suitable for resource-limited outbreak settings due to its simplicity and low cost.
  • The developed assay provides a significant advancement in point-of-care nucleic acid diagnostics.