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Peroxidase Immobilized Cryogels for Phenolic Compounds Removal
Fatma Akpınar1, Sinem Evli1, Gülşen Güven1
1Faculty of Science and Arts, Chemistry Division, Adnan Menderes University, Aydın, Turkey.
Applied Biochemistry and Biotechnology
|July 17, 2019
Summary
This study developed poly(AAm) cryogels for efficient removal of phenolic compounds. The enzyme-modified cryogels demonstrated high peroxidase loading and excellent removal efficiency for various model phenolics.
Area of Science:
- Materials Science
- Biotechnology
- Environmental Science
Background:
- Phenolic compounds are common environmental pollutants.
- Enzyme immobilization is a key strategy for developing efficient removal systems.
- Cryogels offer unique properties for biomolecule immobilization.
Purpose of the Study:
- To synthesize poly(AAm) cryogels.
- To immobilize peroxidase enzyme onto the cryogels.
- To evaluate the efficiency of enzyme-modified cryogels for phenolic compound removal.
Main Methods:
- Cryocopolymerization technique at sub-zero temperatures for poly(AAm) cryogel synthesis.
- Covalent functionalization with peroxidase using EDC/NHS chemistry.
- Characterization using FTIR, SEM, and EDX analysis.
- Kinetic and stability studies of free and immobilized enzymes.
- Phenolic compound removal efficiency testing.
Main Results:
- Poly(AAm) cryogels were successfully synthesized and characterized.
- Maximum peroxidase loading achieved was 127.30 mg/g cryogel.
- Immobilized peroxidase retained significant activity and stability.
- Very high removal efficiency was recorded for model phenolic compounds including phenol, bisphenol A, guaiacol, pyrogallol, and catechol.
Conclusions:
- Poly(AAm) cryogels are effective matrices for peroxidase immobilization.
- Enzyme-modified cryogels show high potential for removing phenolic pollutants from water.
- The developed system offers a promising solution for environmental remediation.
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