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Inhibition of galactosyltransferase activity by cacodylate buffer
F A Boyle1, N D Cook, T J Peters
1Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Abstract:
Cacodylate buffer, frequently used in the assay of galactosyltransferase, causes inhibition of enzyme purified from human pleural effusion. The inhibition is reflected by an increase in the Km (UDP-galactose) and a decrease in Vmax and cannot be reversed by the addition of 2-mercaptoethanol. It is suggested that alternative buffers be used for the study of the enzyme.