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Updated: Jan 22, 2026

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
Published on: December 19, 2013
Dynamics of actin polymerisation during the mammalian single-cell wound healing response
Corina DeKraker1, Laurence Goldin-Blais1, Eric Boucher1
1Department of Anatomy and Cell Biology, Faculty of Medicine, McGill University, 3640 University Street, Strathcona Anatomy and Dentistry Bldg, Montreal, QC, H3A 0C7, Canada.
Objective:
The contribution of actomyosin contractile rings in the wound healing program of somatic cells as never been directly assessed. This contrast with the events characterising the wound healing response of in wounded Xenopus oocytes, in which formation and contraction of an actomyosin ring provides a platform for cytoskeletal repair and drives the restoration of proper plasma membrane composition at the site of injury. As such, we aimed to characterize, using high-resolution live-cell confocal microscopy, the cytoskeletal repair dynamics of HeLa cells.
Results:
We confirm here that the F-actin enrichment that characterizes the late repair program of laser-wounded cells is mostly uniform and is not associated with co-enrichment of myosin-II or the formation of concentric zones of RhoA and Cdc42 activity.
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