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Updated: Jan 22, 2026
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
Published on: November 27, 2014
AgBR1 antibodies delay lethal Aedes aegypti-borne West Nile virus infection in mice
Ryuta Uraki1, Andrew K Hastings1, Doug E Brackney2
11The Anlyan Center for Medical Research and Education, Department of Internal Medicine, Section of Infectious Diseases, Yale University School of Medicine, 300 Cedar Street, New Haven, CT 06520-8031 USA.
Abstract:
West Nile virus (WNV) is transmitted by mosquitoes and can cause severe disease, including meningoencephalitis. AgBR1 is a mosquito salivary protein that enhances Aedes aegypti mosquito-borne Zika virus pathogenesis in mice. Here, we show that AgBR1 antibodies reduce the initial West Nile viral load and delay lethal infection after feeding by an infected Aedes aegypti mosquito. Targeting AgBR1 may therefore be incorporated into strategies to prevent mosquito-transmitted West Nile virus infection.
Insights
Antibodies targeting the AgBR1 mosquito salivary protein reduced West Nile virus (WNV) in mice. This finding suggests AgBR1 as a potential target for preventing WNV infection transmitted by mosquitoes.
Area of Science:
- Virology
- Immunology
- Medical Entomology
Background:
- West Nile virus (WNV) is a mosquito-borne pathogen causing severe neurological disease.
- Mosquito salivary proteins can influence pathogen transmission and host response.
- AgBR1, a protein from *Aedes aegypti* saliva, is known to enhance Zika virus pathogenesis.
Purpose of the Study:
- To investigate the role of AgBR1 in West Nile virus infection.
- To determine if targeting AgBR1 can mitigate WNV pathogenesis.
Main Methods:
- Generation of antibodies against the AgBR1 mosquito salivary protein.
- Experimental infection of mice with WNV-infected *Aedes aegypti* mosquitoes.
- Assessment of viral load and survival rates in treated versus control groups.
Main Results:
- Administration of AgBR1 antibodies significantly reduced the initial West Nile virus load in mice.
- Treatment with AgBR1 antibodies delayed the onset of lethal infection.
- These results indicate a protective effect against WNV infection.
Conclusions:
- AgBR1 antibodies demonstrate efficacy in reducing WNV viral load and delaying lethal outcomes.
- Targeting the AgBR1 protein presents a potential novel strategy for preventing mosquito-borne West Nile virus transmission.
- Further research into AgBR1-based interventions is warranted for public health applications.
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