Related Experiment Video
Updated: Jan 22, 2026

Metagenomic Analysis of Silage
Published on: January 13, 2017
Metagenomics in ophthalmology: Hypothesis or real prospective?
Paola Gallon1, Mohit Parekh2, Stefano Ferrari1
1Fondazione Banca degli Occhi del Veneto, Venice, Italy.
Abstract:
Metagenomic analysis was originally associated with the studies of genetic material from environmental samples. But, with the advent of the Human Microbiome Project, it has now been applied in clinical practices. The ocular surface (OS) is the most exposed part of the eye, colonized by several microbial communities (both, OS and environmental) that contribute to the maintenance of the physiological state. Limited knowledge has been acquired on these microbes due to the limitations of conventional diagnostic methods. Emerging fields of research are focusing on Next Generation Sequencing (NGS) technologies to obtain reliable information on the OS microbiome. Currently only pre-specified pathogens can be detected by conventional culture-based techniques or Polymerase Chain Reaction (PCR), but there are conditions to state whether metagenomics could revolutionize the diagnosis of ocular diseases. The aim of this review is to provide an updated overview of the studies involving NGS technology for OS microbiome.
Related Concept Videos
What is a Hypothesis?
Hypothesis Test for Test of Independence
H0: The two variables (factors)...
Types of Hypothesis Testing
When the null and alternative hypotheses are stated, it is observed that the null hypothesis is a neutral statement against which the alternative hypothesis is tested. The alternative hypothesis is a claim that instead has a certain direction. If the null hypothesis claims that p = 0.5, the alternative hypothesis would be an opposing statement to this and can be put either p > 0.5, p < 0.5, or p...
Errors In Hypothesis Tests
Statistical Hypothesis Testing
Statistical significance measures the probability that an observed result occurred by chance. If this probability, known as...
Facial Feedback Hypothesis

