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Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

Published on: April 14, 2011

Mobile imaging platform for digital influenza virus counting.

Yoshihiro Minagawa¹, Hiroshi Ueno¹, Kazuhito V Tabata¹

¹Department of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Japan. hnoji@appchem.t.u-tokyo.ac.jp.

|July 18, 2019

Summary

A new smartphone-based platform enables sensitive, quantitative digital bioassays for point-of-care diagnostics. This mobile imaging system successfully detected influenza virus in clinical samples, offering a promising tool for rapid disease testing.

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Area of Science:

Biomolecular analysis
Point-of-care diagnostics
Mobile health technology

Background:

Droplet-based digital bioassays offer high sensitivity for biomolecule analysis.
Current digital bioassays rely on bulky fluorescence microscopy, hindering point-of-care applications.

Purpose of the Study:

To develop a compact, smartphone-based mobile imaging platform for digital bioassays.
To demonstrate the platform's capability for quantitative analysis of enzymes and viruses.

Main Methods:

Development of a portable smartphone-based imaging platform (23 × 10 × 7 cm).
Assay of bovine alkaline phosphatase activity.
Digital counting of influenza virus particles using a neuraminidase activity assay.

Main Results:

Successful digital enzyme assay and influenza virus counting.
Observed distinct fluorescence spots from single virus particles.
Demonstrated good linearity between detected spots and virus titer, achieving high sensitivity.
Detected clinical samples with 100x greater sensitivity than commercial rapid tests.

Conclusions:

The smartphone-based platform is suitable for sensitive and quantitative digital bioassays.
This technology shows significant potential for developing point-of-care influenza virus detection systems.
The platform offers a portable and accessible alternative to traditional fluorescence microscopy for diagnostics.