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Dentin Priming with Amphipathic Antimicrobial Peptides.

D G Moussa1, J A Kirihara2, Z Ye1

  • 11 MDRCBB-Minnesota Dental Research Center for Biomaterials and Biomechanics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, USA.

Journal of Dental Research
|July 18, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a new peptide coating for dentin that creates a water-repellent, antibiofilm surface to prevent recurrent caries around dental restorations.

Keywords:
caries detection/diagnostic/preventionimagingmaterials science(s)restorative materialssurface chemistry/properties

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Area of Science:

  • Biomaterials Science
  • Dental Materials
  • Nanotechnology

Background:

  • Bonded restorations have limited lifespans primarily due to recurrent caries at the restoration margins.
  • Degradation of the dentin/restoration interface by water and acids allows bacterial colonization and reinfection.

Purpose of the Study:

  • To develop and evaluate a novel 2-tier protective technology using amphipathic and antimicrobial peptides (AAMPs) to prevent recurrent caries.
  • To investigate the structure-function relationships of AAMPs and their interaction with dentin.

Main Methods:

  • Tested various AAMPs for their ability to form hydrophobic, antibiofilm coatings on dentin.
  • Investigated dentin-conditioning methods to optimize AAMP immobilization.
  • Utilized multiphoton-second harmonic generation imaging to visualize AAMP distribution.
  • Analyzed AAMP release rates in artificial saliva.

Main Results:

  • AAMPs with a high β-sheet content demonstrated superior antimicrobial activity and hydrophobic coating formation on dentin.
  • AAMPs preferentially adsorbed to the mineral phase of dentin.
  • Partial deproteinization of dentin enhanced AAMP immobilization compared to total etching.

Conclusions:

  • Priming dentin with AAMPs offers a versatile approach to fortify vulnerable adhesive interfaces.
  • This technology has the potential to significantly increase the longevity of bonded restorations by resisting recurrent caries.