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PMab-247 Detects Bear Podoplanin in Immunohistochemical Analysis
Junko Takei1,2, Yoshikazu Furusawa1,3, Shinji Yamada1
11Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
Summary
A new monoclonal antibody, PMab-247, effectively detects bear podoplanin (bPDPN) in various tissues. This antibody is valuable for immunohistochemical studies of lung and kidney diseases in bears.
Area of Science:
- Immunohistochemistry
- Molecular Biology
- Veterinary Pathology
Background:
- Podoplanin (PDPN) is a specific marker for type I alveolar cells and lymphatic endothelial cells.
- Sensitive monoclonal antibodies are crucial for PDPN detection in formalin-fixed paraffin-embedded tissues.
- Accurate detection methods are needed for pathophysiological analyses.
Purpose of the Study:
- To evaluate the utility of a newly developed anti-bear PDPN monoclonal antibody (mAb), PMab-247.
- To assess PMab-247's specificity and sensitivity for detecting bear podoplanin (bPDPN).
- To determine the applicability of PMab-247 in immunohistochemical analyses.
Main Methods:
- Development of an anti-bear PDPN monoclonal antibody (mAb), PMab-247.
- Western blot analysis.
- Flow cytometry.
- Immunohistochemical analysis of bear lung and kidney tissues.
Main Results:
- PMab-247 demonstrated strong detection of bPDPN in immunohistochemical analyses.
- bPDPN was confirmed in type I alveolar cells and lymphatic endothelial cells of bear lung.
- bPDPN was also detected in podocytes of bear kidney.
- The antibody showed utility in Western blot and flow cytometry.
Conclusions:
- The anti-bear PDPN mAb, PMab-247, is a sensitive and specific tool for immunohistochemistry.
- PMab-247 is useful for detecting bPDPN in bear lung and kidney tissues.
- This antibody can aid in the pathophysiological analysis of diseases in bears.
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