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Updated: Jan 22, 2026

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds
Published on: June 6, 2015
Structure-activity-distribution relationship study of anti-cancer antimycin-type depsipeptides
Jeremy Seidel1, Yupeng Miao, William Porterfield
1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA. wjzhang@berkeley.edu.
Abstract:
Small-molecule natural products have been an essential source of pharmaceuticals to treat human diseases, but very little is known about their behavior inside dynamic, live human cells. Here, we demonstrate the first structure-activity-distribution relationship (SADR) study of complex natural products, the anti-cancer antimycin-type depsipeptides, using the emerging bioorthogonal Stimulated Raman Scattering (SRS) Microscopy. Our results show that the intracellular enrichment and distribution of these compounds are driven by their potency and specific protein targets, as well as the lipophilic nature of compounds.
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