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Trypsin Pre-Treatment Combined With Growth Factor Functionalized Self-Assembling Peptide Hydrogel Improves Cartilage

Gustavo Zanotto1, Paul Liebesny2, Myra Barrett1

  • 1Department of Clinical Sciences, Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 300 West Drake Road, Fort Collins, CO, 80523.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|July 19, 2019
PubMed
Summary

This study enhanced cartilage repair using a KLD hydrogel combined with growth factors and trypsin pre-treatment. The combined approach improved tissue integration and cellular morphology in rabbit models.

Keywords:
cartilage repairgrowth factorsself-assembling peptide hydrogeltrypsin

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Area of Science:

  • Orthopaedic Research
  • Biomaterials Science
  • Regenerative Medicine

Background:

  • Cartilage defects pose significant challenges in orthopaedics.
  • Current treatments have limitations in promoting effective cartilage repair and integration.
  • Novel biomaterials and growth factor combinations are needed to enhance healing.

Purpose of the Study:

  • To evaluate the efficacy of a self-assembling KLD hydrogel functionalized with growth factors for cartilage repair.
  • To investigate the role of enzymatic trypsin pre-treatment in enhancing cartilage integration.
  • To assess the combined effect of KLD hydrogel, growth factors, and trypsin on osteochondral defect healing.

Main Methods:

  • Osteochondral defects were created in rabbit femoral trochlear grooves.
  • Treatments included KLD hydrogel, growth factors (platelet-derived growth factor-BB and heparin-binding insulin-like growth factor-1), and trypsin pre-treatment.
  • Evaluations involved MRI, radiography, macroscopic, histological, and immunohistochemical analyses at 90 days.

Main Results:

  • Trypsin pre-treatment improved cellular morphology and subchondral bone reconstitution.
  • Platelet-derived growth factor-BB enhanced subchondral bone healing and integration.
  • The combination of growth factors with KLD hydrogel and trypsin pre-treatment led to improved tissue and cell morphology.

Conclusions:

  • Self-assembling KLD hydrogel functionalized with growth factors and trypsin pre-treatment significantly improves cartilage repair.
  • This multi-modal approach offers a promising strategy for treating osteochondral defects.
  • Further research may explore clinical applications of this regenerative technique.