Gene expression during genital morphogenesis in the ground beetle Carabus maiyasanus

To investigate the developmental genetics of genital formation in the carabid beetle Carabus maiyasanus, we compared gene expression patterns among five stages using transcriptomic RNA sequencing data from abdominal segments and genitalia in the third (last) larval instar (including prepupa) and pupal stages. We identified 18 839 genes, of which 10 796 were differentially expressed among stages or between sexes. There were relatively few differentially expressed genes (DEGs) between the sexes (3%). The DEGs were clustered into six groups, mainly according to stage-specific expression patterns. Genes in clusters 1-3 showed high expression levels before pupation and low expression levels during the pupal period, whereas genes in clusters 4-6 showed high expression levels from the prepupal to the pupal stages. Genes related to the initial pupation process and differentiation of genital discs in Drosophila were involved in clusters 4 and 6 and showed low expression levels at early third instar and elevated expression levels from the early prepupal stage, suggesting that the pupation process and genital differentiation started in the prepupal stage. Clusters 4 and 5 included developmental genes related to organ size control, which may be important in the formation of internal genital structures during the pupal stage.