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Complete Genome Sequence of the Novel Klebsiella pneumoniae Phage Marfa

Laith Harb1, Justin Boeckman1,2, Heather Newkirk1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|July 20, 2019
PubMed

Insights

The complete genome of Klebsiella pneumoniae myophage Marfa was sequenced. This T4-like virus shares many proteins with phage T4, offering insights into phage evolution.

Area of Science:

  • Microbiology
  • Genomics
  • Virology

Background:

  • Bacteriophages, or phages, are viruses that infect bacteria.
  • Klebsiella pneumoniae is an opportunistic pathogen.
  • T4-like phages represent a significant group of bacteriophages with diverse hosts.

Purpose of the Study:

  • To present the complete genome sequence of the Klebsiella pneumoniae myophage Marfa.
  • To analyze the genetic makeup and potential functions of Marfa's genes.
  • To compare Marfa with its closest relatives, particularly phage T4.

Main Methods:

  • Whole-genome sequencing of myophage Marfa.
  • Bioinformatic analysis of the genome sequence.
  • Comparative genomics to identify shared genes and proteins.

Main Results:

  • The complete genome sequence of myophage Marfa was determined to be 168,532 base pairs.
  • Marfa's genome contains 289 predicted genes, with functions identified for 122 of them.
  • Significant protein homology was observed between Marfa and phage T4, as well as other related phages.

Conclusions:

  • The genomic data provides a foundation for understanding Marfa's biology and replication.
  • Marfa's genetic similarities to T4 suggest evolutionary relationships within the T4-like phage family.
  • Further research can explore Marfa's specific interactions with Klebsiella pneumoniae.

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