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Updated: Jan 22, 2026
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
Published on: October 17, 2018
Prognostic impact of circulating monocyte subsets in pediatric solid tumors
Asmaa M Zahran1, Amal Rayan2, Heba Abdel Razik Sayed3
1Clinical Pathology Department, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
Insights
In pediatric solid tumors, intermediate and non-classical monocytes were linked to better treatment responses. Non-classical monocytes also showed a positive correlation with overall survival in these young patients.
Area of Science:
- Immunology
- Pediatric Oncology
- Flow Cytometry
Background:
- Monocyte subsets play crucial roles in immune responses.
- Understanding monocyte subset dynamics in pediatric cancers is vital for treatment optimization.
Purpose of the Study:
- To quantify monocyte subsets in newly diagnosed pediatric solid tumors.
- To investigate the association between monocyte subsets and treatment outcomes in pediatric cancer patients.
Main Methods:
- Prospective case-controlled study involving 100 pediatric solid tumor patients and 40 healthy controls.
- Blood samples collected pre-chemotherapy analyzed for monocyte subsets using flow cytometry.
- Routine laboratory tests performed alongside monocyte subset assessment.
Main Results:
- Significant elevations in intermediate and non-classical monocytes were observed in pediatric cancer patients compared to controls.
- Non-classical and intermediate monocytes demonstrated a significant impact on treatment response.
- A positive correlation was found between non-classical monocytes and overall survival (OS).
Conclusions:
- Non-classical and intermediate monocytes are associated with improved treatment response in pediatric solid tumors.
- Non-classical monocytes correlate with higher overall survival.
- Further research is necessary to fully elucidate the functional roles of monocyte subsets in diverse pediatric tumors.
Background And Aim:
We aimed to quantify monocyte subsets in newly diagnosed pediatric patients with solid tumors at South Egypt Cancer Institute (SECI) and Assiut University Hospital (AUH), and investigate their roles in the treatment outcomes.
Patients And Methods:
This is a prospective case-controlled study included 100 patients with de novo solid tumors and forty age and sex matched healthy children to provide blood samples as control subjects to determine normal count of monocyte subsets, blood samples were collected from cancer patients before the first cycle of chemotherapy, these blood samples were subjected to routine laboratory tests and assessment of monocyte subsets using flow cytometry.
Results:
Significant accumulations of intermediate monocytes and non classical monocytes (P< 0.000) in pediatric cases compared to controls were detected, there was a significant impact of non classical and intermediate monocytes on the type of response (P< 0.008, P< 0.4 respectively), The median OS for 100 patients with pediatric solid tumors involved in our study was 27 ± 0.589 months with 95% CI = 25.846-28.154, while the median PFS was 26 ± 0.610 months with 95% CI = 24.805-27.195, significant positive correlation between non-classical monocytes and OS (r=+0.659, P< 0.041).
Conclusion:
Solid conclusion regarding the impact of monocyte classes in pediatric tumors is premature, although, in this study, non-classical and intermediate monocytes were associated with better response to treatment in pediatric solid tumors and non-classical monocytes were correlated with higher overall survival; further studies are needed for better understanding and specification of monocyte functions in different pediatric tumors.
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