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Infectious cDNA clones of two strains of Mayaro virus for studies on viral pathogenesis and vaccine development

Christina Chuong1, Tyler A Bates1, James Weger-Lucarelli1

  • 1Department of Biomedical Sciences and Pathobiology, Virginia Tech, VA-MD Regional College of Veterinary Medicine, Blacksburg, VA, USA.

Virology
|July 21, 2019
PubMed

Insights

Researchers developed infectious clones for Mayaro virus (MAYV), an emerging threat. These tools enable studying MAYV

Area of Science:

  • Virology
  • Molecular Biology
  • Public Health

Background:

  • Mayaro virus (MAYV) is an emerging alphavirus with severe clinical manifestations.
  • Existing molecular tools are insufficient for understanding MAYV transmission and pathogenesis.
  • MAYV shares similarities with other significant arboviruses like Zika, dengue, and chikungunya.

Purpose of the Study:

  • To develop and characterize infectious clones of Mayaro virus (MAYV).
  • To create a molecular resource for studying MAYV virulence, transmission, and vaccine development.

Main Methods:

  • Development of infectious cDNA clones for two MAYV strains.
  • Characterization of clone-derived viruses in mammalian (CD-1 mice) and mosquito cell lines.
  • Assessment of viral replication, infection rates, and phenotypes.

Main Results:

  • Infectious MAYV was produced from the developed clones.
  • Clone-derived viruses replicated similarly to wild-type MAYV in mammalian and mosquito cells.
  • Infection rates and phenotypes of clone-derived viruses matched parental strains in mice.

Conclusions:

  • The developed infectious clone system is a valuable resource for MAYV research.
  • This system will facilitate analysis of MAYV genetic determinants of virulence.
  • It will aid in determining MAYV vector competence and in developing novel vaccines.
Keywords:
AlphavirusInfectious clonesMayaro virus

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