Serial block face-scanning electron microscopy for volume electron microscopy.

Area of Science:

• Life Science Research
• Microscopy Techniques
• Nanotechnology

Background:

• Serial Block Face Scanning Electron Microscopy (SBF-SEM) is increasingly used for 3D imaging in life sciences.
• Originally developed for neuroscience, its application has broadened to other research areas.
• SBF-SEM enables the capture of 3D volumes from monolayers to multiple tissue layers with nanometer resolution.

Purpose of the Study:

• To describe the Serial Block Face Scanning Electron Microscopy (SBF-SEM) workflow.
• To identify and explain adjustable steps within the SBF-SEM sample preparation process.
• To facilitate the adaptation of SBF-SEM for various sample types in life science research.

Main Methods:

• Detailed description of the Serial Block Face Scanning Electron Microscopy (SBF-SEM) workflow.
• Identification of critical steps in sample preparation amenable to modification.
• Methodology focused on optimizing SBF-SEM for diverse biological specimens.

Main Results:

• A comprehensive overview of the SBF-SEM process is provided.
• Specific steps within the workflow that can be adjusted for different samples are highlighted.
• The study offers insights into tailoring SBF-SEM protocols for broader applicability.

Conclusions:

• The SBF-SEM technique is a powerful tool for high-resolution 3D imaging in life sciences.
• Understanding and adapting specific workflow steps is crucial for successful SBF-SEM implementation across different research domains.
• This work provides a valuable resource for researchers seeking to optimize SBF-SEM for their unique sample requirements.