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Updated: Jan 21, 2026

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
Published on: November 13, 2017
Genome-wide localization of the polyphenol quercetin in human monocytes
Dana Atrahimovich1,2, Avraham O Samson2, Yifthah Barsheshet2
1Department of Oxidative Stress and Human Diseases, MIGAL - Galilee Research Institute, 11016, Kiryat Shmona, Israel.
Background:
Quercetin is a polyphenol of great interest given its antioxidant activity and involvement in the immune response. Although quercetin has been well studied at the molecular level as a gene regulator and an activator of specific cellular pathways, not much attention has been given to its mechanism of action at the genome-wide level. The present study aims to characterize quercetin's interaction with cellular DNA and to show its subsequent effect on downstream transcription.
Results:
Two massive parallel DNA-sequencing technologies were used: Chem-seq and RNA-seq. We demonstrate that upon binding to DNA or genome-associated proteins, quercetin acts as a cis-regulatory transcription factor for the expression of genes that are involved in the cell cycle, differentiation and development.
Conclusions:
Such findings could provide new and important insights into the mechanisms by which the dietary polyphenol quercetin influences cellular functions.
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