Related Experiment Video
Updated: Jan 21, 2026

Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes
Published on: January 10, 2022
Immunoassay for rapid on-site detection of glyphosate herbicide
E Viirlaid1, M Ilisson2, S Kopanchuk2
1Institute of Chemistry, University of Tartu, Ravila 14A, 50411, Tartu, Estonia. edith.viirlaid@ut.ee.
Abstract:
Glyphosate is the most widespread herbicide and its global use is steadily increasing. Although glyphosate is considered to have low toxicity, its wide application has raised concerns about its effects on human health. The extensive use of glyphosate has risen a need of its continuous monitoring in drinking and surface waters to assure in accordance with the set standards. Within the present study, we have developed a novel assay for the on-site detection of glyphosate by combining flow-through technology with the high specificity of immunorecognition. The proposed biosensing system was based on the detection of fluorescence signal generated by the quantitative replacement of glyphosate in antigen-antibody complex with IgY-type anti-glyphosate antibodies on microbeads by synthetic 5-carboxytetramethylrhodamine (5-TAMRA) conjugated glyphosate. The working range of this assay was in low millimolar range and the time required for glyphosate detection around 0.5 h. The applicability of the immunoassay for glyphosate detection in surface water was tested and the biosensor results were validated with high-performance liquid chromatography.
More Related Videos
Related Concept Videos
Plant Breeding and Biotechnology
Rapidly Varying Flow
RACE - Rapid Amplification of cDNA Ends
Plant Tissue Culture
Detection of Gross Error: The Q Test
Detection of Black Holes
Their closest cousins are neutron stars, which are composed almost entirely of neutrons packed against each other, making them extremely dense. A neutron star has the same mass as the Sun but its diameter is only a few kilometers. Therefore, the escape velocity from their surface is close to the speed of light.
Not until the 1960s, when the first neutron...

