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Related Concept Videos

Factors Affecting Solubility04:01

Factors Affecting Solubility

36.7K
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Chȃtelier’s principle. Consider the dissolution of silver iodide:
36.7K
Solubility Equilibria03:07

Solubility Equilibria

56.8K
Solubility equilibria are established when the dissolution and precipitation of a solute species occur at equal rates. These equilibria underlie many natural and technological processes, ranging from tooth decay to water purification. An understanding of the factors affecting compound solubility is, therefore, essential to the effective management of these processes. This section applies previously introduced equilibrium concepts and tools to systems involving dissolution and precipitation.
The...
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Physical Properties Affecting Solubility02:19

Physical Properties Affecting Solubility

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Solutions of Gases in Liquids
As for any solution, the solubility of a gas in a liquid is affected by the attractive intermolecular forces between solute and solvent species. Unlike solid and liquid solutes, however, there is no solute-solute intermolecular attraction to overcome when a gaseous solute dissolves in a liquid solvent since the atoms or molecules comprising a gas are far separated and experience negligible interactions. Consequently, solute-solvent interactions are the sole...
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Solubility of Ionic Compounds02:55

Solubility of Ionic Compounds

68.0K
Solubility is the measure of the maximum amount of solute that can be dissolved in a given quantity of solvent at a given temperature and pressure. Solubility is usually measured in molarity (M) or moles per liter (mol/L). A compound is termed soluble if it dissolves in water.
68.0K
Solubility03:00

Solubility

21.0K
Solution, Solubility, and Solubility Equilibrium
A solution is a homogeneous mixture composed of a solvent, the major component, and a solute, the minor component. The physical state of a solution—solid, liquid, or gas—is typically the same as that of the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
In a solution, the solute particles (molecules,...
21.0K
Chemical and Solubility Equilibria02:21

Chemical and Solubility Equilibria

4.9K
The free energy change associated with dissolving a solute in a liter of solvent is called the free energy of a solution, ΔGsolution. The overall ΔGsolution is expressed as the balance of ΔGinteraction against the always-favorable free-energy of mixing, ΔGmixing. Solution formation is favorable if  ΔGsolution is less than zero, whereas it is unfavorable if ΔGsolution is greater than zero. In short, for a solution to form and complete dissolution to take place,...
4.9K

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Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
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Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro

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Measuring Protein Solubility.

Neer Asherie1,2

  • 1Department of Physics, Yeshiva University, New York, NY, USA. asherie@yu.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 26, 2019
PubMed
Summary
This summary is machine-generated.

This study measured thaumatin protein solubility with tartrate ions at varying temperatures. The findings provide a method for determining protein solubility in concentrated solutions.

Keywords:
Phase diagramsProteinProtein crystallizationSolubilityThaumatin

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Area of Science:

  • Biochemistry
  • Protein Chemistry

Background:

  • Protein solubility is crucial for applications using concentrated protein solutions.
  • Understanding solubility parameters is key for protein formulation and processing.

Purpose of the Study:

  • To measure the solubility of the protein thaumatin.
  • To investigate the effect of tartrate ions on protein solubility.
  • To determine solubility as a function of temperature.

Main Methods:

  • Solubility measurements were performed on the protein thaumatin.
  • The influence of tartrate ions on solubility was assessed.
  • Experiments were conducted across a range of temperatures.

Main Results:

  • Thaumatin solubility was successfully measured in the presence of tartrate ions.
  • A functional relationship between temperature and thaumatin solubility was established.

Conclusions:

  • The study presents a viable method for measuring protein solubility.
  • This method is applicable to other proteins and can aid in optimizing concentrated protein solutions.