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3D printing of step-gradient nanocomposite hydrogels for controlled cell migration
Andisheh Motealleh1, Betül Çelebi-Saltik2,3, Nihal Ermis1,4
1Physikalisches Institut and Center for Soft Nanoscience, Westfälische Wilhelms-Universität Münster, Busse-Peus-Strasse 10, 48149 Münster, Germany.
Biofabrication
|July 26, 2019
Summary
Researchers developed a 3D printed gradient nanocomposite hydrogel that guides cell migration and enhances bone cell differentiation. This novel biomaterial promotes healing without specialized growth media, utilizing silica-based nanomaterials.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Nanotechnology
Background:
- Developing advanced hydrogels for regenerative medicine is crucial.
- Controlling cell behavior within engineered scaffolds remains a challenge.
- Nanomaterials offer unique properties for biomaterial applications.
Purpose of the Study:
- To create a step-gradient nanocomposite (NC) hydrogel using 3D printing.
- To investigate the hydrogel's ability to direct cell migration.
- To evaluate the potential for enhanced osteogenic differentiation of stem cells.
Main Methods:
- Fabrication of gradient NC hydrogels by 3D printing varying nanomaterial concentrations.
- Assessment of self-adhesive properties.
- In vitro studies using fibroblast and human bone marrow-derived mesenchymal stem cells (hBM MSC).
Main Results:
- The 3D printed gradient NC hydrogel successfully directed fibroblast cell migration towards higher nanomaterial concentrations.
- Enhanced migration and osteogenic differentiation of hBM MSC were observed.
- Osteogenic differentiation occurred without specific induction media, attributed to silica-based nanomaterials.
- Increased nanomaterial content promoted cell migration and calcium deposition.
Conclusions:
- Step-gradient NC hydrogels fabricated via 3D printing are promising for regenerative applications.
- The gradient structure effectively guides cell migration.
- Silica-based nanomaterials within the hydrogel promote stem cell differentiation and mineralization.
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