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A Combination of Muramylpeptides from Gram-Negative Bacteria Corrects Hematological and Immunological Disorders

M V Kiselevskii1, N Yu Anisimova2, S M Sitdikova2

  • 1Laboratory of Cellular Immunity, N. N. Blokhin National Medical Research Center, Ministry of Health of the Russian Federation, Moscow, Russia. kisele@inbox.ru.

Bulletin of Experimental Biology and Medicine
|July 27, 2019
PubMed

Insights

Polyramyl, a natural muramylpeptide combination, effectively restored immune cell counts and spleen health in mice experiencing cyclophosphamide-induced immunosuppression. This study highlights polyramyl

Area of Science:

  • Immunology
  • Pharmacology
  • Microbiology

Background:

  • Cyclophosphamide-induced immunosuppression in C57Bl/6 mice leads to reduced leukocyte counts and altered T cell subpopulations.
  • Immunosuppression negatively impacts spleen morphology, specifically depleting the white pulp cellular composition.
  • Natural muramylpeptides, like polyramyl, are investigated for their immunomodulatory potential.

Purpose of the Study:

  • To evaluate the therapeutic effect of polyramyl on immune recovery following cyclophosphamide-induced immunosuppression.
  • To assess the impact of polyramyl on circulating T cell subpopulations (CD4+ T cells) and the CD4/CD8 ratio.
  • To examine the restoration of spleen morphology and blood leukocyte levels by polyramyl.

Main Methods:

  • C57Bl/6 mice were administered cyclophosphamide to induce immunosuppression.
  • Polyramyl, a combination of natural muramylpeptides, was administered subcutaneously.
  • Flow cytometry was used to analyze T cell subpopulations, and spleen morphology was assessed.

Main Results:

  • Cyclophosphamide treatment significantly reduced blood leukocyte counts and CD4+ T cells, and damaged splenic white pulp.
  • Polyramyl administration effectively restored blood leukocyte levels and normalized spleen morphology.
  • Polyramyl showed a tendency to increase CD4+ T cells and the CD4/CD8 ratio in immunosuppressed mice.

Conclusions:

  • Polyramyl demonstrates significant immunorestorative properties in a mouse model of cyclophosphamide-induced immunosuppression.
  • The compound effectively reverses key hematological and histological changes associated with immunosuppression.
  • Polyramyl shows potential as an immunomodulatory agent for managing drug-induced immune deficits.
Keywords:
cyclophosphamideimmunomodulationimmunosuppressionmuramylpeptidespolymuramyl

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