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Mass Spectrometry for Proteomics-Based Investigation.

Alisa G Woods1, Izabela Sokolowska1, Armand G Ngounou Wetie1

  • 1Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA.

Advances in Experimental Medicine and Biology
|July 27, 2019
PubMed
Summary

Mass spectrometry (MS) and proteomics have advanced, enabling identification beyond proteins to include modifications, isoforms, and interactions. This review covers MS/proteomics principles and strategies for comprehensive biological analysis.

Keywords:
LC-MS/MSMALDI-MSMass spectrometryProteomics

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Mass spectrometry (MS) and proteomics have seen significant advancements in instrumentation, fractionation, and bioinformatics.
  • Current technology demands more than just protein identification, including characterization of post-translational modifications (PTMs), isoforms, truncations, protein-protein interactions (PPIs), and quantitation.

Purpose of the Study:

  • To describe the principles of MS and proteomics.
  • To outline strategies for identifying proteins, PTMs, isoforms, truncations, PPIs, and protein quantitation.
  • To discuss the strengths and weaknesses of current MS and proteomics methodologies.

Main Methods:

  • Review of mass spectrometry principles.
  • Discussion of various proteomics strategies for protein and PTM identification.
  • Analysis of techniques for PPI, isoform, and truncation detection.
  • Exploration of protein quantitation methods.

Main Results:

  • Detailed explanation of MS and proteomics principles.
  • Comprehensive overview of strategies for identifying proteins, PTMs, isoforms, truncations, PPIs, and quantitation.
  • Discussion of the strengths and limitations within the field.

Conclusions:

  • Mass spectrometry and proteomics are crucial for detailed biological analysis.
  • Future applications in scientific and clinical settings are assessed.
  • This chapter serves as an introduction to advanced MS proteomic methodologies and related fields like lipidomics and metabolomics.