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Related Concept Videos

Proteomics01:33

Proteomics

9.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Protein-protein Interfaces02:04

Protein-protein Interfaces

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Protein and Protein Structure02:15

Protein and Protein Structure

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Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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What are Proteins?01:55

What are Proteins?

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Overview
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Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

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Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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Proteomics and Non-proteomics Approaches to Study Stable and Transient Protein-Protein Interactions.

Armand G Ngounou Wetie1, Izabela Sokolowska2, Devika Channaveerappa2

  • 1Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA. ngounoa@clarkson.edu.

Advances in Experimental Medicine and Biology
|July 27, 2019
PubMed
Summary

Proteomics and other methods identify protein interactions, crucial for understanding cellular functions and diseases. This study explores techniques for analyzing stable and transient protein-protein interactions (PPIs).

Keywords:
Mass spectrometryProtein-protein interactionsProteomics

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Proteomics

Background:

  • Human genome encodes ~2% of proteins, yet ~1-2 million protein entities exist.
  • Protein diversity arises from alternative splicing, post-translational modifications (PTMs), and protein-protein interactions (PPIs).
  • Understanding protein functions and roles in disease requires studying these complex interactions.

Purpose of the Study:

  • To explore proteomics and non-proteomics approaches for studying protein-protein interactions.
  • To differentiate between stable and transient protein complexes.
  • To provide insights into biological processes and disease mechanisms.

Main Methods:

  • Proteomics-based strategies for protein identification, sequencing, and quantification.
  • Non-proteomics techniques to investigate protein interactions.
  • Analysis of both stable and transient protein-protein interactions (PPIs).

Main Results:

  • Proteomics enables comprehensive identification and quantification of proteins and their modifications.
  • Various methods can distinguish between stable and transient protein complexes.
  • These approaches are vital for mapping cellular machinery.

Conclusions:

  • Studying protein-protein interactions (PPIs) is essential for understanding cellular functions and disease.
  • Both proteomics and non-proteomics methods offer valuable insights into PPIs.
  • Further research into PPIs will advance biological and medical sciences.