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Updated: Jan 21, 2026

Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis
Published on: December 9, 2022
Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry
Akiyoshi Hirayama1, Satsuki Ikeda2, Asako Sato2
1Institute for Advanced Biosciences, Keio University, Yamagata, Japan. hirayama@ttck.keio.ac.jp.
Abstract:
Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.
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