Related Experiment Video
Updated: Jan 21, 2026
08:13
Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
Published on: September 13, 2011
28.1K
Monophasic action potential amplitude for substrate mapping
Ikeotunye Royal Chinyere1,2, Mathew Hutchinson1,3, Talal Moukabary4
1Sarver Heart Center, University of Arizona, Tucson, Arizona.
Summary
Bipolar voltage amplitude (BVA) mapping accurately quantified infarct size in a heart failure model, outperforming monophasic action potential amplitude (MAPA) mapping. BVA mapping is recommended for research and clinical electrophysiology.
Area of Science:
- Cardiovascular Electrophysiology
- Cardiac Mapping
- Heart Failure Research
Background:
- Radiofrequency ablation is effective for tachyarrhythmias but suffers from recurrence.
- Accurate substrate identification is crucial for successful arrhythmia ablation.
- Bipolar voltage electrogram sensitivity can be limited by its narrow field of view.
Purpose of the Study:
- To investigate the accuracy of monophasic action potential amplitude (MAPA) mapping for infarct size quantification compared to other modalities.
- To compare MAPA, MAP duration to 90% repolarization (MAPD90), unipolar voltage amplitude (UVA), and bipolar voltage amplitude (BVA) mapping against histopathology.
- To determine the optimal mapping technique for distinguishing healthy myocardium from scar tissue in ischemic heart failure.
Main Methods:
- Utilized a rat model of ischemic heart failure induced by left coronary artery ligation.
- Performed in vivo ventricular epicardial mapping using MAPA, MAPD90, UVA, and BVA.
- Compared mapping data with gold standard histopathological measurements of infarct size.
Main Results:
- MAPD90 and UVA effectively discriminated healthy myocardium from scar tissue (P < 0.0158).
- MAPA and BVA showed significant decreases between healthy, border, and scar tissues (P < 0.0218).
- BVA mapping demonstrated the highest accuracy in quantifying infarct size, contrary to the initial hypothesis.
Conclusions:
- Bipolar voltage amplitude (BVA) mapping is superior for infarct size quantification in this model.
- Monophasic action potential amplitude (MAPA) mapping, while having high spatial resolution, was less accurate quantitatively.
- BVA mapping's utility is reinforced for translational research and clinical electrophysiology, with potential for MAPA in characterizing fibrosis-related diseases like atrial fibrillation.
More Related Videos
11:20Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning
Published on: June 2, 2014
12.4K
09:26Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart
Published on: September 10, 2015
9.7K
Related Concept Videos
01:31Action Potential
4.4K
Neurons communicate by firing action potentials—the electrochemical signal that is propagated along the axon. The signal results in the release of neurotransmitters at axon terminals, thereby transmitting information to the nervous system. An action potential is a specific "all-or-none" change in membrane potential that results in a rapid spike in voltage.
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
4.4K
01:14Action Potential
10.8K
Neurons communicate by firing action potentials—the electrochemical signal that is propagated along the axon. The signal results in the release of neurotransmitters at axon terminals, thereby transmitting information to the nervous system. An action potential is a specific "all-or-none" change in membrane potential that results in a rapid spike in voltage.
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
10.8K
01:41Action Potentials
141.6K
Overview
141.6K
01:23Propagation of Action Potentials
9.1K
The propagation of an action potential refers to the process by which a nerve impulse, or "action potential," travels along a neuron.
Neurons (nerve cells) have a resting membrane potential, with a slightly negative charge inside compared to outside. This is maintained by ion channels, such as sodium (Na+) and potassium (K+) channels, which control the flow of ions. When a stimulus, like a touch or a signal from another neuron, triggers the neuron, sodium channels open, allowing sodium ions to...
Neurons (nerve cells) have a resting membrane potential, with a slightly negative charge inside compared to outside. This is maintained by ion channels, such as sodium (Na+) and potassium (K+) channels, which control the flow of ions. When a stimulus, like a touch or a signal from another neuron, triggers the neuron, sodium channels open, allowing sodium ions to...
9.1K
01:30Cardiac Action Potential
6.0K
Cardiac action potentials are essential for proper heart function, enabling the rhythmic contractions needed for adequate blood circulation. Nodal cells and Purkinje fibers, specialized for electrical conduction, generate these action potentials.
The cardiac action potential process involves a series of phases characterized by the movement of ions across the cardiac cell membranes, leading to the depolarization and repolarization of the cardiac myocytes.
Ionic Basis of Cardiac Action Potentials
The cardiac action potential process involves a series of phases characterized by the movement of ions across the cardiac cell membranes, leading to the depolarization and repolarization of the cardiac myocytes.
Ionic Basis of Cardiac Action Potentials
6.0K
01:28Action Potential: Phases of Stimulation
11.7K
The action potential is a complex electrical event that occurs in excitable cells, such as neurons and muscle cells. It consists of several distinct phases, each with specific characteristics.
Resting Phase:
In this phase, the cell's membrane is at its resting potential, typically around -70 millivolts (mV) for neurons. Inside the cell, there is a higher concentration of potassium ions (K+) and a lower concentration of sodium ions (Na+). Voltage-gated sodium channels are closed, and...
Resting Phase:
In this phase, the cell's membrane is at its resting potential, typically around -70 millivolts (mV) for neurons. Inside the cell, there is a higher concentration of potassium ions (K+) and a lower concentration of sodium ions (Na+). Voltage-gated sodium channels are closed, and...
11.7K