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Related Experiment Videos

K-Sol corneal preservation at room temperature.

K Tamaki1, E D Varnell, H E Kaufman

  • 1Lions Eye Research Laboratories, Louisiana State University Medical Center, School of Medicine, New Orleans.

The British Journal of Ophthalmology
|May 1, 1988
PubMed
Summary

K-Sol medium effectively preserves corneas at room temperature for at least six days, maintaining endothelial cell integrity and organelle structure. This offers a promising alternative to traditional cold storage for corneal tissue.

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Area of Science:

  • Ophthalmology
  • Tissue Engineering
  • Biomaterials Science

Background:

  • Corneal storage media are crucial for maintaining tissue viability before transplantation.
  • Current methods often require refrigeration, limiting accessibility and transport options.
  • K-Sol, a novel medium containing chondroitin sulfate in tissue culture medium (TC 199), has shown promise for extended preservation.

Purpose of the Study:

  • To evaluate the efficacy of K-Sol for corneal tissue storage at room temperature (25°C).
  • To compare K-Sol's performance against M-K medium under ambient conditions.
  • To assess the structural integrity and cellular health of corneas stored in K-Sol.

Main Methods:

  • Rabbit corneas were preserved in K-Sol and M-K medium for 3 and 7 days at 25°C.

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  • Human corneas were stored in K-Sol at 25°C for 6 days.
  • Corneal tissues were analyzed using light microscopy, scanning electron microscopy, and assessment of medium pH.
  • Ultrastructural changes in endothelial cells, mitochondria, and organelles were evaluated.

    • Main Results:

    • Endothelial sheets remained intact after 3 days in both media.
    • K-Sol exhibited less pH change than M-K medium after 7 days.
    • Corneas stored in K-Sol showed organized cytoplasmic organelles and nuclei, unlike those in M-K medium.
    • Human corneas stored in K-Sol for 6 days maintained 95% endothelial integrity with well-preserved organelles.

    Conclusions:

    • K-Sol demonstrates superior stability compared to M-K medium at room temperature.
    • K-Sol is effective for preserving both rabbit and human corneas at 25°C for at least 6 days.
    • This storage method offers a viable alternative for ambient temperature corneal preservation.