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Published on: October 6, 2017
Enhanced Activity of Variant DNA Polymerase β (D160G) Contributes to Cisplatin Therapy by Impeding the Efficiency of
Meina Wang1, Enjie Li1, Lin Lin1
1Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Abstract:
Cisplatin, commonly used in a variety of cancer treatments, induces apoptosis in cancer cells by causing lethal DNA damage. Several DNA repair pathways participate in regulation of cisplatin treatment, leading to cisplatin sensitivity or resistance in cancer cells. DNA polymerase β (pol β), a key protein involved in base excision repair, confers a response to cisplatin therapy that is dependent on polymerase activity. Pol β D160G mutation with enhanced polymerase activity, previously identified in clear cell renal cell carcinoma, enhances the sensitivity of human cancer cells and mouse xenografts to cisplatin by limiting the efficiency of nucleotide excision repair (NER). Notably, the D160G mutation impedes the recruitment of XPA to cisplatin-induced sites of DNA damage, leading to unrepaired damage and further inducing cell death. Molecular architecture analysis indicated that the D160G mutation alters protein-DNA interactions and the surface electrostatic properties of the DNA-binding regions, resulting in greater DNA affinity and polymerase activity compared with wild-type pol β. Collectively, these results indicate that enhancing pol β activity impedes the efficiency of NER and provide a promising adjuvant therapeutic strategy for cisplatin chemotherapy. IMPLICATIONS: Our studies demonstrate that polβ D160G mutation with enhanced polymerase activity impedes NER efficiency during the repair of cisplatin-induced DNA damage, leading to increased cisplatin sensitivity in cancer cells.
Insights
Enhanced DNA polymerase β activity, due to the D160G mutation, increases cancer cell sensitivity to cisplatin chemotherapy by hindering DNA repair pathways like nucleotide excision repair (NER). This finding offers a potential new therapeutic strategy.
Area of Science:
- Molecular Biology
- Cancer Research
- Genetics
Background:
- Cisplatin is a widely used chemotherapy drug that induces DNA damage in cancer cells.
- Cancer cells can develop resistance or sensitivity to cisplatin through various DNA repair pathways.
- DNA polymerase β (pol β) plays a crucial role in DNA repair and influences response to cisplatin therapy.
Purpose of the Study:
- To investigate the impact of the pol β D160G mutation on cancer cell sensitivity to cisplatin.
- To elucidate the molecular mechanisms by which the D160G mutation affects DNA repair and cisplatin response.
Main Methods:
- Analysis of pol β D160G mutation in human cancer cells and mouse xenografts.
- Assessment of nucleotide excision repair (NER) efficiency and XPA recruitment to DNA damage sites.
- Molecular architecture and protein-DNA interaction analysis.
Main Results:
- The pol β D160G mutation enhances polymerase activity and increases sensitivity to cisplatin.
- This mutation impedes the efficiency of NER by hindering XPA recruitment to cisplatin-induced DNA damage.
- Structural analysis revealed altered DNA-binding affinity and electrostatic properties in the D160G mutant.
Conclusions:
- Enhanced pol β activity, specifically the D160G mutation, compromises NER efficiency, leading to increased cisplatin-induced DNA damage and cell death.
- The D160G mutation presents a potential target for enhancing cisplatin chemotherapy efficacy.
- Modulating pol β activity could be a promising adjuvant strategy for cancer treatment.
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