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Updated: Jan 21, 2026

Super-resolution Imaging of Neuronal Dense-core Vesicles
Published on: July 2, 2014
Modulation and study of photoblinking behavior in dye doped silver-silica core-shell nanoparticles for localization
Chumki Chakraborty1, S Thompson2, Veronica J Lyons2
1Department of Mechanical Engineering, Texas Tech University, Lubbock, TX 79409, United States of America.
None:
Blinking of fluorescent nanoparticles is a compelling phenomenon with widely debated mechanisms. The ability to inhibit or control blinking is important for applications in the field of optical, semiconductor and fluorescent imaging. Self-blinking nanomaterials are also attractive labels for localization-based super-resolution microscopy. In this work, we have synthesized silver core silica nanoparticles (Ag@SiO2) doped with Rhodamine 110 and studied the parameters that affect blinking. We found that under nitrogen rich conditions the nanoparticles shifted towards higher duty cycles. Also, it was found that hydrated nanoparticles showed a less drastic response to nitrogen rich conditions as compared to dried nanoparticles, indicating that surrounding matrix played a role in the response of nanoparticles to molecular oxygen. Further, the blinking is not a multi-body phenomena, super-resolution localization combined with intensity histogram analysis confirmed that single particles are emitting.
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