Phylogenetic and Developmental Constraints Dictate the Number of Cusps on Molars in Rodents

Mammal tooth morphology and function correlate strongly with dietary ecology, and convergence is a major feature of mammalian tooth evolution. Yet, function and ecology are insufficient to explain morphological diversification and convergence within mammalian molar evolution; suggesting that development and phylogeny also limit possible structural solutions to selective pressures. Here, I use in silico models and empirical studies of extant and fossil rodent teeth to identify morphogenetic rules that influence molar morphology. Because rodents are the most diverse group of mammals with corresponding dental disparity they represent an excellent system for investigating how genetic interactions limit morphology. I find that lower first molars are limited to a minimum of four cusps and a maximum of nine cusps. Multiple developmental pathways produce the same numbers of cusps, despite highly variable cusp morphologies, indicating the existence of limits on how morphological evolution can fill a morphospace defined by cusp numbers. These constraints are both developmental and phylogenetic in nature and the identification of their influence on rodent molar shape provides a framework for investigation of how tooth batteries evolved an array of functions despite fundamental structural limits. The data presented here increase predictability of cusp number and evolutionary outcomes of rodent cheek dentition.