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Related Experiment Video

Updated: Jan 21, 2026

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions
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Response Regulators 9 and 10 Negatively Regulate Salinity Tolerance in Rice.

Wei-Chen Wang1, Te-Che Lin1, Joseph Kieber2

  • 1Department of Agronomy, National Taiwan University, Roosevelt Road, Taipei, Taiwan.

Plant & Cell Physiology
|July 31, 2019
PubMed
Summary

Rice genes OsRR9 and OsRR10 negatively regulate salinity stress response. Mutants lacking these genes show enhanced salt tolerance by altering ion transporter and photosynthesis gene expression.

Keywords:
Oryza sativaCRISPR/Cas9Cytokinin signalingIon transporterSalt stress

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Cytokinins regulate plant growth and abiotic stress responses.
  • Cytokinin signaling involves two-component systems and type-A response regulators (RRs) that provide negative feedback.
  • Understanding specific RRs in crop plants like rice is crucial for improving stress tolerance.

Purpose of the Study:

  • To investigate the function of two highly similar rice type-A RR genes, OsRR9 and OsRR10.
  • To determine the role of OsRR9 and OsRR10 in salinity stress response in rice.

Main Methods:

  • Generating loss-of-function mutants for OsRR9 and OsRR10 (osrr9/osrr10).
  • Phenotypic analysis of wild type and mutant rice seedlings under salinity stress.
  • Transcriptomic analysis (RNA sequencing) to identify differentially expressed genes.

Main Results:

  • OsRR9 and OsRR10 are induced by cytokinin; only OsRR10 is repressed by salinity.
  • osrr9/osrr10 mutants exhibit significantly higher salinity tolerance than wild type.
  • Upregulation of ion transporter genes (e.g., OsHKT1;1, OsHKT1;3, OsHKT2;1) and genes related to photosynthesis, transcription, and phytohormone signaling in mutants.

Conclusions:

  • OsRR9 and OsRR10 act as negative regulators of salinity stress response in rice.
  • Disruption of OsRR9 and OsRR10 enhances salt tolerance by modulating ion homeostasis and other stress-related pathways.