Related Experiment Video
Updated: Jan 21, 2026

Author Spotlight: Developing Synthetic Microbial Communities for Generating Second-Generation Biofertilizers
Published on: May 24, 2024
Growth Media for Mixed Multispecies Oropharyngeal Biofilm Compositions on Silicone
Matthias Leonhard1, Beata Zatorska1, Doris Moser2
1Department of Otorhinolaryngology, Medical University of Vienna, Waehringer Gürtel 18-20, 1090 Vienna, Austria.
Aims:
Microbial colonization of silicone voice prostheses by bacteria and Candida species limits the device lifetime of modern voice prostheses in laryngectomized patients. Thus, research focuses on biofilm inhibitive properties of novel materials, coatings, and surface enhancements. Goal of this in vitro study was the evaluation of seven commonly used growth media to simulate growth of mixed oropharyngeal species as mesoscale biofilms on prosthetic silicone for future research purposes.
Methods And Results:
Yeast Peptone Dextrose medium (YPD), Yeast Nitrogen Base medium (YNB), M199 medium, Spider medium, RPMI 1640 medium, Tryptic Soy Broth (TSB), and Fetal Bovine Serum (FBS) were used to culture combined mixed Candida strains and mixed bacterial-fungal compositions on silicone over the period of 22 days. The biofilm surface spread and the microscopic growth showed variations from in vivo biofilms depending on the microbial composition and growth medium.
Conclusion:
YPD and FBS prove to support continuous in vitro growth of mixed bacterial-fungal oropharyngeal biofilms deposits over weeks as needed for longterm in vitro testing with oropharyngeal biofilm compositions.
Significance And Impact Of Study:
The study provides data on culture conditions for mixed multispecies biofilm compositions that can be used for future prosthesis designs.
Related Concept Videos
Microbial Growth Media
Suctioning the Oropharyngeal Airway
After assembling the equipment, the nurse should practice hand hygiene and don appropriate PPE according to infection control guidelines to avoid the...
Classifying Matter by Composition
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures.
A pure substance is a form of matter that has a constant composition throughout with uniform properties. For example, any sample of sucrose has the same composition and same physical properties, such as melting point, color, and sweetness, regardless of the source from which it is isolated.
A mixture is composed of two or...
Biofilms
Mixing Concrete
Mixing Time

