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Updated: Jan 21, 2026
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
Published on: September 9, 2014
Zfat Is Indispensable for the Development of Erythroid Cells in the Fetal Liver
Keiko Doi1,2, Toshiyuki Tsunoda3,2, Midori Koyanagi3,2
1Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan kdoi@fukuoka-u.ac.jp.
Background/Aim:
In mice, fetal liver is the first tissue of definitive erythropoiesis for definitive erythroid expansion and maturation. ZFAT, originally identified as a candidate susceptibility gene for autoimmune thyroid disease, has been reported to be involved in primitive hematopoiesis and T cell development. The aim of this study was to examine whether or not Zfat is involved in definitive erythropoiesis in the fetal liver during mammalian development.
Materials And Methods:
The role of Zfat during mouse fetal erythropoiesis in the fetal liver was examined using tamoxifen-inducible CreERT2 Zfat-deficient mice.
Results:
Zfat-deficient mice exhibit moderate anemia with small and pale fetal liver through a decreased number of erythroblasts by E12.5. Apoptosis sensitivity in fetal liver erythroid progenitors was enhanced by Zfat-deficiency ex vivo. Moreover, Zfat knockdown partially inhibited CD71-/lowTer119- to CD71highTer119- transition of fetal liver erythroid progenitors with impairment in the elevation of CD71 expression.
Conclusion:
Zfat plays a critical role for erythropoiesis in the fetal liver.
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