Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple Ig classes on rabbit B lymphocytes.

B J Bast, R Manten-Slingerland, R E Ballieux

    Advances in Experimental Medicine and Biology
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Immunodiagnostics in birmingham.

    Immunology today·2014
    Same author

    Co-operative T-T interactions in the human T suppressor cell circuit.

    Immunology today·2014
    Same author

    Hypofibronectinaemia and severity of sickle cell anaemia in Nigeria.

    British journal of haematology·2001
    Same author

    Study of the mechanisms of killing of Mycobacterium bovis BCG by apoptosis in J774 murine macrophages.

    JPMA. The Journal of the Pakistan Medical Association·2000
    Same author

    Efficacy of an anti-CD138 immunotoxin and doxorubicin on drug-resistant and drug-sensitive myeloma cells.

    International journal of cancer·1999
    Same author

    Immune responses to experimental stress: effects of mental effort and uncontrollability.

    Psychosomatic medicine·1999
    Same journal

    Mammalian Respiratory Chain Complex Assemblies and Their Links to Mitochondria Stress-Induced Human Diseases.

    Advances in experimental medicine and biology·2026
    Same journal

    Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.

    Advances in experimental medicine and biology·2026
    Same journal

    The Pyruvate Dehydrogenase Complex: A 90-Year-Old Enigma Shaping the Future of Structural Enzymology.

    Advances in experimental medicine and biology·2026
    Same journal

    Regulation of the Anti-termination RNA Transcription Complex by Lon-Mediated Lambda N Degradation.

    Advances in experimental medicine and biology·2026
    Same journal

    PCNA Macromolecular Complexes: PCNA Serves as a Molecular Hub Regulating Multiple Cellular Processes Inside and Outside of the Nucleus.

    Advances in experimental medicine and biology·2026
    Same journal

    Dynamic Assemblies in Genome Maintenance.

    Advances in experimental medicine and biology·2026
    See all related articles

    This study investigated B cell surface immunoglobulin (s-Ig) isotypes in rabbits. Findings suggest B cell differentiation involves sequential expression of IgM, IgG, and IgA, with potential IgD presence.

    Area of Science:

    • Immunology
    • Cell Biology
    • Veterinary Science

    Background:

    • B lymphocytes (B cells) express surface immunoglobulins (s-Ig) crucial for adaptive immunity.
    • Understanding B cell differentiation and isotype expression is vital for immunological research.
    • Rabbit peripheral blood lymphocytes (PBL) were used to study s-Ig classes.

    Purpose of the Study:

    • To characterize the presence and distribution of different s-Ig classes on rabbit B cells.
    • To investigate the endogenous origin of these immunoglobulin proteins.
    • To explore potential B cell differentiation pathways based on isotype expression.

    Main Methods:

    • Flow cytometry was employed to detect various immunoglobulin isotypes on rabbit lymphocytes.
    • Specific antibody conjugates targeting different immunoglobulin classes and allotypes were utilized.

    Related Experiment Videos

  • Experimental conditions ensured the endogenous origin of detected s-Ig.

    • Main Results:

    • Approximately 40% of rabbit lymphocytes were identified as B cells (Fab and a1 positive in homozygous rabbits).
    • IgG-positive lymphocytes were detected using an anti-allotype conjugate, while anti-Fc gamma conjugates were negative.
    • Most B cells expressed IgM, with many co-expressing IgG or IgA; no lymphocytes expressed both IgG and IgA simultaneously.
    • Analysis suggested the potential presence of IgD-positive lymphocytes.

    Conclusions:

    • Rabbit B cell differentiation appears to involve sequential expression of immunoglobulin isotypes.
    • The findings provide insights into the B cell repertoire and maturation process in rabbits.
    • Further investigation is warranted to confirm the presence and role of IgD on rabbit B cells.