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Evaluation of an In-House Colistin NP Test for Use in Resource-Limited Settings.
B Mitton1,2, C Kingsburgh3, M M Kock1,2
1Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.
Journal of Clinical Microbiology
|August 2, 2019
Summary
The colistin NP test accurately detects colistin resistance in Gram-negative bacteria, showing high agreement with the gold standard. This rapid, inexpensive method is ideal for resource-limited settings.
Area of Science:
- Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Colistin is crucial for treating multidrug-resistant Gram-negative bacterial infections.
- Global emergence of colistin resistance necessitates reliable detection methods.
- The colistin NP test offers a potential rapid screening assay.
Purpose of the Study:
- To evaluate the performance of an in-house colistin NP test.
- To compare the colistin NP test against broth microdilution (BMD) and Etest for colistin resistance detection.
- To assess the accuracy of the colistin NP test in identifying colistin resistance.
Main Methods:
- Evaluated 215 stored *Enterobacteriaceae* isolates using the colistin NP test.
- Compared colistin NP test results with broth microdilution (BMD) as the gold standard.
- Compared colistin NP test results with Etest as an alternative method.
Main Results:
- The colistin NP test demonstrated 98.1% categorical agreement with BMD.
- Etest showed 87.9% categorical agreement with BMD.
- The colistin NP test had a very major error rate of 1.9% compared to 15.7% for Etest.
Conclusions:
- The colistin NP test performs satisfactorily compared to the BMD reference method.
- The colistin NP test is a rapid, inexpensive, and accurate screening method for colistin resistance.
- The colistin NP test is particularly valuable in resource-limited settings.
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