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Molecular network-based identification of competing endogenous RNAs in bladder cancer

Wei-Dong Jiang1, Ping-Cheng Yuan1

  • 1Department of Urology and Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, China.

Plos One
|August 2, 2019
PubMed
Abstract

Insights

Circular RNAs (circRNAs) act as competitive endogenous RNAs (ceRNAs) in bladder cancer (BC). This study constructed a circRNA-miRNA-mRNA network, identifying potential therapeutic biomarkers for BC pathogenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Bioinformatics

Background:

  • Circular RNAs (circRNAs) function as competitive endogenous RNAs (ceRNAs), regulating gene expression and impacting tumorigenesis.
  • The specific role of circRNA-ceRNA networks in bladder cancer (BC) pathogenesis is largely unknown.
  • Understanding these mechanisms is crucial for identifying novel therapeutic targets in BC.

Purpose of the Study:

  • To elucidate the regulatory mechanisms of circRNA-mediated ceRNAs in bladder cancer (BC).
  • To construct a comprehensive circRNA-miRNA-mRNA ceRNA network specific to BC.
  • To identify potential diagnostic and therapeutic biomarkers for bladder cancer.

Main Methods:

  • Utilized RNA expression profiles from public datasets (GEO, TCGA) to build a circRNA-miRNA-mRNA network.
  • Analyzed protein-protein interactions using the STRING database and identified hub genes with cytoHubba.
  • Performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses on differentially expressed mRNAs.

Main Results:

  • Identified 27 circRNAs, 76 microRNAs (miRNAs), and 4744 messenger RNAs (mRNAs) with differential expression between BC and normal tissues.
  • Constructed a BC-specific ceRNA network comprising 21 circRNAs, 14 miRNAs, and 150 mRNAs.
  • Discovered 10 hub genes and enriched pathways including homologous recombination and Fanconi anemia, suggesting roles in BC development.

Conclusions:

  • Screened dysregulated circRNAs and established a circRNA-associated ceRNA network through bioinformatics analysis.
  • The identified ceRNAs are implicated in the pathogenesis of bladder cancer.
  • These circRNAs represent promising candidates for future therapeutic biomarkers in BC treatment.

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