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Non-Invasive Electrical Brain Stimulation Montages for Modulation of Human Motor Function
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Adseverin modulates morphology and invasive function of MCF7 cells.
Biochimica Et Biophysica Acta. Molecular Basis of Disease
|August 2, 2019
Summary
Adseverin (Ads) protein influences cancer cell shape and matrix invasion. Deleting Ads reduced cell extensions and collagen degradation, impacting cancer cell metastasis.
Area of Science:
- Cell Biology
- Cancer Research
- Biochemistry
Background:
- Adseverin (Ads) is a Ca2+-dependent actin-binding protein.
- High Ads expression is observed in gastric, prostate, and bladder cancers.
- The role of Ads in cancer cell actin remodeling, extension formation, and matrix invasion remains unclear.
Purpose of the Study:
- To investigate the role of Adseverin (Ads) in subcortical actin remodeling.
- To determine Ads' contribution to cell extension formation and matrix invasion in cancer cells.
Main Methods:
- CRISPR/Cas9 was used to generate Adseverin wildtype and Ads-null MCF7 breast cancer cells.
- Cell morphology, extension formation, and matrix degradation were compared between wildtype and Ads-null cells.
- Cell migration and collagen degradation assays were performed on 2D and 3D matrices.
Main Results:
- Ads-null cells showed significantly reduced cell extension formation (37% on fibronectin) and a more circular morphology.
- Reconstitution of Ads restored cell extension formation in Ads-null cells.
- Ads deletion reduced cell invasion through Transwell membranes (~40%) and collagen degradation (including MT1-MMP expression and phagocytosis).
Conclusions:
- Adseverin plays a crucial role in cell extension formation and collagen degradation in MCF7 breast cancer cells.
- These Ads-dependent processes may influence cancer cell matrix invasion and metastasis.
- Targeting Adseverin could be a potential therapeutic strategy for certain cancers.
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