Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

10.8K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
10.8K
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

14.9K
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
14.9K
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

10.0K
10.0K
Bone Remodeling01:40

Bone Remodeling

40.3K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
40.3K
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

4.0K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
4.0K
Ligand Binding Sites02:40

Ligand Binding Sites

14.9K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A guide to using embedded ethics in human stem-cell-based embryo model research.

Nature cell biology·2026
Same author

The emerging importance of UBA6 in ubiquitylation and proteostasis.

Nature reviews. Molecular cell biology·2026
Same author

Proximity labeling reveals non-catalytic interactions between DPP9 and ubiquitin signaling complexes.

Cellular and molecular life sciences : CMLS·2026
Same author

The GT1 domain of RNase J ensures RNA quality control through dsRNA binding in Arabidopsis plastids.

Nucleic acids research·2026
Same author

Combination of CBD with minor cannabinoid CBDV suppresses CXCR4 via CB2 receptor and alleviates colitis in mice.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Viral NblA proteins negatively affect oceanic cyanobacterial photosynthesis.

Nature·2025

Related Experiment Video

Updated: Jan 21, 2026

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

3.1K

Remodeling Membrane Binding by Mono-Ubiquitylation.

Neta Tanner1, Oded Kleifeld2, Iftach Nachman1

  • 1School of Neurobiology, Biochemistry and Biophysics, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

Biomolecules
|August 3, 2019
PubMed
Summary

Ubiquitin receptor ubiquitylation, specifically on yeast epsin, dissociates it from membranes. This allows receptors to bind new substrates, promoting cyclic activity in ubiquitylation signaling.

Keywords:
Ub receptorphosphatidylinositol phosphateubiquitylation

More Related Videos

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

Published on: June 10, 2020

6.0K
Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

8.3K

Related Experiment Videos

Last Updated: Jan 21, 2026

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

3.1K
Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

Published on: June 10, 2020

6.0K
Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

8.3K

Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Ubiquitin (Ub) receptors are key mediators of ubiquitylation signals, binding ubiquitylated substrates.
  • Ubiquitin receptors themselves undergo dynamic ubiquitylation and deubiquitylation cycles.
  • The functional significance of ubiquitin receptor ubiquitylation remains largely unexplored.

Purpose of the Study:

  • To investigate the function of ubiquitin receptor ubiquitylation.
  • To determine the role of ubiquitylation in the activity of yeast epsin, a ubiquitin receptor involved in endocytosis.

Main Methods:

  • Mass spectrometry to identify ubiquitylation sites on yeast epsin.
  • In vitro binding assays using purified ubiquitylated epsin and liposomes.
  • In vivo studies using live cell imaging to observe epsin behavior upon mimicked ubiquitylation.

Main Results:

  • Lysine-3 was identified as the major ubiquitylation site in the epsin plasma membrane binding domain.
  • Ubiquitylated epsin showed reduced binding to phosphatidylinositol-4,5-bisphosphate (PIP2)-enriched liposomes compared to non-ubiquitylated epsin.
  • Mimicking ubiquitylation in vivo caused epsin dissociation from the plasma membrane.

Conclusions:

  • Ubiquitylation of yeast epsin at lysine-3 disrupts its membrane association by competing with PIP2 binding.
  • This dissociation allows ubiquitin receptors to detach from their products, enabling binding to new ubiquitylated substrates.
  • Ubiquitin receptor ubiquitylation facilitates a cyclic mechanism, promoting sustained signaling and receptor turnover.