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Updated: Jan 21, 2026

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
Published on: April 5, 2021
Data of H2O2 release from AQP8-knockdown rat hepatocyte mitochondria
Mauro Danielli1, Julieta Marrone1, Alejo M Capiglioni1
1Instituto de Fisiología Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570, 2000, Rosario, Santa Fe, Argentina.
Abstract:
This article reports experimental data related to the research article entitled "Mitochondrial aquaporin-8 is involved in SREBP-controlled hepatocyte cholesterol biosynthesis" [Danielli et al., 2019]. We present data about hydrogen peroxide (H2O2) release from mitochondria isolated from rat hepatocytes with or without silencing of aquaporin-8 (AQP8) protein expression. The rate of mitochondrial H2O2 release (pmoles/min/mg mitochondrial protein) was found to be decreased by about 50% in AQP8-knockdown mitochondria.
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