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The stomach comprises several layers that work together to facilitate digestion and protect the organ. The outermost layer is called the serosa, which provides support and protection to the stomach. The muscularis externa layer is responsible for the mechanical breakdown of food by contracting and moving the stomach. The submucosa layer, located beneath the muscularis externa, contains connective tissue, blood vessels, nerves, and glands that secrete mucus and other substances essential for...
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The microscopic anatomy of the liver is a complex and intricate system that comprises numerous structural units known as liver lobules, each of which is comparable in size to a sesame seed. These hexagonal structures consist of plates of liver cells or hepatocytes, which are characterized by their versatility and abundance of cellular apparatus like rough and smooth ER, Golgi apparatus, peroxisomes, and mitochondria.
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A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
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A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
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Troubleshooting in the histology laboratory.

Clifford M Chapman1

  • 1Medi-Sci Consultants , Woburn , MA , USA.

Journal of Histotechnology
|August 6, 2019
PubMed
Summary

Histology errors are inevitable due to human and machine limitations. Understanding the underlying theories of histology procedures and stains, coupled with a problem-solving approach, is key to effective troubleshooting and corrective actions.

Keywords:
H&E stainingTroubleshootingfixationhistologymicrotomyspecimen receiptsub-optimaltissue processing

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Area of Science:

  • Histopathology
  • Laboratory Science
  • Quality Management

Background:

  • Human and machine error rates contribute to sub-optimal events in histology.
  • Histology procedures and staining techniques are complex and prone to errors.
  • Effective troubleshooting is crucial for maintaining diagnostic accuracy in histopathology.

Purpose of the Study:

  • To provide a comprehensive review of troubleshooting strategies in the histology laboratory.
  • To elucidate the theoretical underpinnings of common histology procedures and stains.
  • To present a systematic problem-solving methodology for addressing histology-related errors.

Main Methods:

  • Review of existing literature on histology techniques and error analysis.
  • Explanation of theoretical frameworks for key histology procedures.
  • Outline of a structured problem-solving approach for laboratory issues.

Main Results:

  • Identification of inherent error sources in human and machine performance.
  • Detailed explanation of the scientific principles behind various histology stains.
  • A framework for root cause analysis and implementation of corrective actions.

Conclusions:

  • Understanding the theory behind histology methods is fundamental for troubleshooting.
  • A systematic problem-solving approach enhances the reliability of histology results.
  • Implementing evidence-based corrective actions minimizes the impact of errors in histopathology.