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Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells
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CelLock TM: an innovative standardized cell-block preparation procedure.

Clifford M Chapman1

  • 1Medi-Sci Consultants, Woburn, MA, USA.

Journal of Histotechnology
|March 10, 2022
PubMed
Summary

The CelLock™ kit standardizes cell-block preparation from various specimens, preserving cellular integrity for histology and molecular testing. This method ensures reproducible embedding and sectioning for comprehensive analysis.

Keywords:
CelLGel™CelLock™biopsycell-blockcytologyfine needle aspirationformalin-fixed paraffin-embeddedhematoxylin and eosinhistologyin situ hybridizationmolecular pathologyspecial stains immunohistochemistry

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Area of Science:

  • Histopathology
  • Cytopathology
  • Biotechnology

Background:

  • Cellular specimens like fine needle aspirates (FNA) and small biopsies often present challenges for traditional processing.
  • Existing methods may not preserve all cellular material or genetic information effectively for downstream analysis.
  • Standardized protocols are needed for optimal cell-block preparation.

Purpose of the Study:

  • To introduce and evaluate the CelLock™ procedure kit for collecting and preparing diverse cellular specimens into paraffin cell-blocks.
  • To demonstrate the utility of CelLock™-prepared cell-blocks for subsequent histological and molecular analyses.
  • To establish a standardized method for reproducible cell-block processing.

Main Methods:

  • The CelLock™ kit was used to process various cellular specimens, including FNA, cultured cells, and small biopsies.
  • Prepared cell-blocks were embedded and sectioned using standard microtomy techniques.
  • Sections were subjected to hematoxylin and eosin (H&E) staining, special stains, immunohistochemistry (IHC), and in situ hybridization (ISH).

Main Results:

  • The CelLock™ procedure successfully created paraffin cell-blocks from a wide range of cellular specimens, including those with scant tissue or cells.
  • Virtually all submitted specimens were contained within the cell-blocks, allowing for reproducible embedding and sectioning.
  • The preserved cellular material within the cell-blocks retained protein and genetic information suitable for histological and ancillary testing.

Conclusions:

  • The CelLock™ procedure kit offers a standardized and effective method for preparing cell-blocks from challenging cellular specimens.
  • This method ensures optimal preservation of cellular material for comprehensive histological, immunohistochemical, and molecular analyses.
  • CelLock™ facilitates reproducible processing, enhancing the diagnostic yield from small or scant specimens.