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Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril
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Sensitive polydopamine bi-functionalized SERS immunoassay for microalbuminuria detection.

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  • 1Department of Chemistry, Fudan University, Shanghai, 200438, China.

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|August 6, 2019
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Summary

This study presents a new, affordable method using polydopamine (PDA) and surface-enhanced Raman scattering (SERS) for detecting albumin. This sensitive SERS immunoassay offers accurate results for diabetic nephropathy and cardiovascular disease markers.

Keywords:
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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Immunosensing

Background:

  • Albumin is a crucial prognostic indicator in diabetic nephropathy and cardiovascular disease.
  • Accurate and sensitive detection of albumin is vital for disease management.
  • Existing methods may have limitations in sensitivity, cost, or sample volume.

Purpose of the Study:

  • To develop a rapid, sensitive, and inexpensive method for albumin detection.
  • To utilize polydopamine (PDA) functionalized glass chips and surface-enhanced Raman scattering (SERS) tags for immunoassay.
  • To establish a reliable diagnostic tool for albuminuria.

Main Methods:

  • Development of a bi-functionalized glass chip using polydopamine (PDA) for antibody immobilization.
  • Utilizing SERS tags with anti-albumin antibodies for sandwich immunoassay formation.
  • Optimization of experimental conditions for enhanced sensitivity and specificity.

Main Results:

  • Achieved a wide linear detection range of 10-300 mg/L for albumin.
  • Demonstrated a low limit of detection (LOD) of 0.2 mg/L.
  • Exhibited high specificity against common proteins like hemoglobin (Hgb) and immunoglobulin G (IgG).
  • Validated results against the clinical standard immunoturbidimetric method using patient urine samples.
  • Required minimal sample volume (2 μL).

Conclusions:

  • The developed SERS immunoassay is accurate, sensitive, and requires minimal sample volume.
  • This method offers a superior alternative to traditional dipstick and immunoturbidimetric assays for albumin detection.
  • The PDA-SERS approach provides a promising tool for early diagnosis and monitoring of kidney and cardiovascular diseases.