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One-step sensitive thrombin detection based on a nanofibrous sensing platform
Xiaoling Li1, Yuyang Wu1, Jingjing Niu1
1College of Chemistry, Sichuan University, Chengdu 610064, P. R. China. zcs@scu.edu.cn.
Journal of Materials Chemistry. B
|August 7, 2019
Summary
This study presents a novel one-step method for detecting protein biomarkers using a nanofibrous sensing platform. The technique offers a sensitive, label-free approach for rapid disease diagnosis and point-of-care testing.
Area of Science:
- Biomarker Detection
- Nanomaterials Science
- Biosensing Technology
Background:
- Rapid disease diagnosis relies on efficient detection of ultralow concentrations of protein biomarkers.
- Existing methods often lack convenience and speed, hindering point-of-care applications.
- Developing one-step, sensitive detection strategies is crucial for early disease identification.
Purpose of the Study:
- To develop a convenient and time-saving one-step detection method for protein biomarkers.
- To utilize a nanofibrous sensing platform combined with proximity-induced DNA strand displacement (PiDSD), catalytic hairpin assembly (CHA), and thioflavin T (ThT) binding.
- To optimize interface behaviors for enhanced reaction kinetics and thermodynamics on the nanofibrous membrane.
Main Methods:
- Fabrication of a nanofibrous sensing platform.
- Integration of proximity-induced DNA strand displacement (PiDSD) for signal generation.
- Incorporation of catalytic hairpin assembly (CHA) for signal amplification.
- Utilizing thioflavin T (ThT) binding for sensitive detection of the target biomarker (thrombin).
Main Results:
- Achieved a limit of detection (LOD) as low as 1.0 pM for thrombin.
- Demonstrated a wide linear detection range from 50 pM to 5 nM.
- Exhibited excellent specificity and good long-term stability of the nanofibrous sensing platform.
- The one-step method proved to be label-free and highly sensitive.
Conclusions:
- The developed one-step nanofibrous sensing platform offers a highly sensitive and convenient method for protein biomarker detection.
- This approach shows significant advantages over previous one-step thrombin detection methods, being label-free and more sensitive.
- The platform holds strong potential for applications in point-of-care testing (POCT) and early disease diagnosis.
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