An electrochemiluminescence immunosensor for the N-terminal brain natriuretic peptide based on the high quenching...

A sandwich-type electrochemiluminescence (ECL) immunosensor for the N-terminal brain natriuretic peptide (NT-proBNP) is described. The assay is based on the quenching of the ECL of graphite-like carbon nitride (g-C₃N₄) by polydopamine (PDA). Two-dimensional g-C₃N₄ is grown in-situ on titanium dioxide nanoflowers (TiO₂ NFs). The macroporous structure of the NFs enhances the interfacial stability of g-C₃N₄, and also promotes the ECL reaction of g-C₃N₄ with the co-reactant. The introduction of gold nanoparticles into the matrix further enhances the ECL and facilitates the immobilization of capture antibodies. The nanoquencher used to label the secondary antibody is synthesized by catalytic polymerization of dopamine in the presence of bimetallic NiPd nanoparticles. The nanoquencher preserves the high reactivity of polydopamine and quenches the ECL of the g-C₃N₄/TiO₂ system. Compared to other methods, the detection limit for NT-proBNP is decreased to 50 fg∙mL^-1. Graphical abstract Schematic presentation of the electrochemiluminescence (ECL) process of the immunosensor: titanium dioxide nanoflowers@graphite-like carbon nitride-gold nanoparticles (TiO₂ NFs@g-C₃N₄-Au) as luminophor, and polydopamine (PDA) as nanoquencher.