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Coiled Optical Nanofiber for Optofluidic Absorbance Detection.

Hongyan Mei1, Jing Pan1, Zhang Zhang1

  • 1State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering , Zhejiang University , Hangzhou 310027 , China.

ACS Sensors
|August 7, 2019
PubMed
Summary

This study introduces a coiled optical nanofiber sensor for highly sensitive optofluidic absorbance detection. The novel sensor achieves a low detection limit for chemicals and biological analytes, outperforming standard methods.

Keywords:
absorbance detectionchloramphenicol sensingenzyme-linked immunosorbent assaymicrofluidicsoptical nanofiber

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Area of Science:

  • Optofluidics
  • Nanotechnology
  • Chemical Sensing

Background:

  • Optofluidic absorbance detection faces limitations due to short optical path lengths, leading to high detection limits.
  • Developing sensitive and robust detection methods is crucial for various analytical applications.

Purpose of the Study:

  • To introduce a novel optofluidic absorbance detection concept using a coiled optical nanofiber.
  • To enhance sensitivity and achieve lower detection limits in optofluidic sensing.

Main Methods:

  • Utilizing a coiled optical nanofiber within a microfluidic channel for enhanced light-matter interaction.
  • Measuring the absorbance of ferric chloride (FeCl3) solutions to assess sensor performance.
  • Demonstrating chloramphenicol sensing using the enzyme-linked immunosorbent assay (ELISA) method.

Main Results:

  • The sensor achieved a detection limit as low as 10 μM for FeCl3 solutions within a 0-5 mM range.
  • Sensitivity was 10-fold higher compared to standard absorbance measurements using a 1 cm cuvette.
  • Achieved a detection limit below 0.5 ng/L for chloramphenicol using ELISA.

Conclusions:

  • The coiled optical nanofiber design significantly enhances sensitivity and reduces detection limits in optofluidic sensing.
  • The use of a polydimethylsiloxane (PDMS) pillar ensures excellent reversibility and sensor robustness.
  • This technology holds promise for developing ultrasensitive chemical and biological sensing platforms.