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A novel hydrazide compound exerts anti-metastatic effect against breast cancer

Soudeh Dehghani1, Zahra Kooshafar1, Ali Almasirad2

  • 1Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.

Biological Research
|August 8, 2019
PubMed
Abstract

Insights

A novel hydrazide derivative, compound B, effectively inhibits breast cancer metastasis in vitro and in vivo. This compound shows significant potential as an anti-metastatic agent for drug-resistant breast cancer.

Area of Science:

  • Oncology
  • Pharmacology
  • Cancer Biology

Background:

  • Metastatic breast cancer presents significant treatment challenges due to resistance to existing therapies.
  • Novel anticancer agents are crucial for overcoming treatment barriers, particularly cancer cell spread.
  • Two hydrazide derivatives demonstrated anti-cancer activity in breast cancer cells, prompting further investigation.

Purpose of the Study:

  • To evaluate the therapeutic potential of N'-((5-nitrothiophen-2-yl)methylene)-2-(phenylthio)benzohydrazide (compound B) against metastatic breast cancer.
  • To assess the anti-metastatic effects of compound B in both in vitro and in vivo models.
  • To explore compound B's efficacy in overcoming resistance to current chemotherapeutics.

Main Methods:

  • In vitro assessment of MDA-MB-231 cell invasion using Matrigel assay and MMP-9 expression analysis.
  • In vivo study involving inoculation of 4T1 mammary carcinoma cells in BALB/c mice.
  • Intraperitoneal administration of compound B for 4 weeks, followed by immunohistochemistry for proliferation index and angiogenesis.

Main Results:

  • Compound B significantly inhibited MDA-MB-231 cell invasion in vitro, reducing MMP-9 expression.
  • Daily administration of compound B suppressed primary tumor growth and lung metastasis in a 4T1 breast cancer mouse model.
  • Immunohistochemical analysis revealed reduced proliferation and microvessel density in tumors and lungs of treated mice.

Conclusions:

  • Compound B exhibits significant anti-metastatic activity in preclinical breast cancer models.
  • The novel hydrazide derivative demonstrates potential as a therapeutic agent for metastatic breast cancer.
  • Further research into compound B could lead to new treatments for drug-resistant metastatic breast cancer.
Keywords:
Breast cancerImmunohistochemistryMatrigelMetastasisSoft agar

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