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Published on: February 5, 2014
Phylogenetic characterization of rhinoviruses from infants in Sarlahi, Nepal
Jane Kuypers1, Garrett A Perchetti1, Helen Y Chu2
1Department of Laboratory Medicine, University of Washington, Seattle, Washington.
Insights
Rhinoviruses (RVs) are common in infants in Nepal, with RV-A being most frequent. All three RV species were detected, but none were linked to more severe respiratory illness in infants.
Area of Science:
- Virology
- Pediatric Infectious Diseases
- Molecular Epidemiology
Background:
- Rhinoviruses (RVs) are a leading cause of acute respiratory infections in infants and young children.
- Genetic diversity of RVs complicates understanding their epidemiology and impact on health.
Purpose of the Study:
- To characterize the diversity of Rhinovirus (RV) species and types causing respiratory illness in infants in Nepal.
- To investigate the association between RV species and clinical presentation or severity.
Main Methods:
- Infants in Nepal were monitored via household surveillance for respiratory symptoms.
- Nasal swabs from RV-positive infants were sequenced to identify RV species and types.
- Clinical data on symptoms and medical care were collected.
Main Results:
- Rhinovirus A (RV-A), RV-B, and RV-C were detected in 68%, 6%, and 26% of sequenced samples, respectively.
- 94 unique RV types were identified, with RV-A being the most common.
- No significant differences in symptoms, pneumonia, or medical care-seeking were observed across RV species.
Conclusions:
- All three RV species and numerous types circulate in young infants in rural Nepal.
- RV-A is the predominant species detected in this population.
- Rhinovirus species were not associated with differences in respiratory illness severity in infants.
Problem:
Rhinoviruses (RVs), the most common causes of acute respiratory infections in young children and infants, are highly diverse genetically.
Objective:
To characterize the RV types detected with respiratory illness episodes in infants in Nepal.
Study Methods:
Infants born to women enrolled in a randomized trial of maternal influenza immunization in rural, southern Nepal were followed with household-based weekly surveillance until 180 days of age. Infants with respiratory symptoms had nasal swabs tested for twelve respiratory viruses. A subset with RV alone was selected for sequencing of the VP4/2 gene to identify RV types.
Results:
Among 547 RV-only positive illnesses detected from December 2012 to April 2014, 285 samples (52%) were sequenced. RV-A, B, and C species were detected in 193 (68%), 18 (6%), and 74 (26%) specimens, respectively. A total of 94 unique types were identified from the sequenced samples, including 52 RV-A, 11 RV-B, and 31 RV-C. Multiple species and types circulated simultaneously throughout the study period. No seasonality was observed. The median ages at illness onset were 88, 104, and 88 days for RV-A, B, and C, respectively. The median polymerase chain reaction cycle threshold values did not differ between RV species. No differences between RV species were observed for reported respiratory symptoms, including pneumonia, or for medical care-seeking.
Conclusions:
Among very young, symptomatic infants in rural Nepal, all three species and many types of RV were identified; RV-A was detected most frequently. There was no association between RV species and disease severity.
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