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Updated: Jan 21, 2026

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A Novel Theranostic Nanoprobe for In Vivo Singlet Oxygen Detection and Real-Time Dose-Effect Relationship Monitoring

Han Wang1, Zhaohui Wang1, Yongkuan Li1

  • 1State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China.

Small (Weinheim an Der Bergstrasse, Germany)
|August 8, 2019
PubMed

Insights

This study introduces a novel nanoplatform for real-time singlet oxygen detection during cancer photodynamic therapy (PDT). This innovation allows for evaluating the dose-effect relationship and achieving tumor treatment simultaneously.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Photodynamic Therapy

Background:

  • Singlet oxygen is crucial for cancer photodynamic therapy (PDT) but challenging to detect in real-time within living systems.
  • Accurate monitoring of singlet oxygen is needed to establish the dose-effect relationship for optimizing PDT efficacy.

Purpose of the Study:

  • To develop a Förster resonance energy transfer (FRET)-based upconversion nanoplatform for in vivo singlet oxygen monitoring.
  • To evaluate the in vivo dose-effect relationship between singlet oxygen and PDT efficacy.
  • To achieve simultaneous tumor treatment with the developed nanoplatform.

Main Methods:

  • Constructed a nanoplatform using core-shell upconversion nanoparticles (UCNPs) as donors, IR-820 as an acceptor, MC540 photosensitizer, and PAA-octylamine.
  • Utilized FRET mechanism for energy transfer between UCNPs and IR-820.
  • Monitored singlet oxygen levels via luminescence intensity changes of UCNPs at 800 nm emission during PDT in tumor tissues.

Main Results:

  • The nanoplatform sensitively reflected singlet oxygen levels generated in tumor tissues during PDT.
  • Demonstrated the ability to evaluate the in vivo dose-effect relationship between singlet oxygen and PDT efficacy.
  • Achieved satisfactory tumor treatment with good biocompatibility.

Conclusions:

  • This work presents the first theranostic nanoplatform capable of in vivo singlet oxygen monitoring, dose-effect evaluation, and tumor treatment.
  • The developed strategy offers a new approach for assessing PDT efficacy based on singlet oxygen pathways.
  • The nanoplatform shows potential for advancing cancer theranostics and personalized PDT.
Keywords:
detectiondose-effect relationshipsinglet oxygenupconversion nanoparticles

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