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Generation and isolation of cyanobacterial inside-out thylakoid vesicles
F Nilsson1, D J Simpson, A C Stewart
1Department of Biochemistry, Arrhenius Laboratories, University of Stockholm, Sweden.
Archives of Biochemistry and Biophysics
|September 1, 1988
Abstract:
A method has been designed to prepare inside-out thylakoid vesicles from a cyanobacterial species (Phormidium laminosum). Everted thylakoid vesicles could be generated by Yeda press treatment after induced membrane pairing. Membrane pairing was induced either by addition of high concentrations of Mg2+ ions or by lowering the pH of the fragmentation media. The inside-out vesicles were isolated by aqueous polymer two-phase partition. The membrane orientation was determined by proton translocation studies and freeze-fracture electron microscopy.