Related Experiment Video

Updated: Jan 21, 2026

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

875

MiR-126 promotes endothelial cell apoptosis by targeting PI3K/Akt in rats with lower limb arteriosclerosis obliterans

W-M Li1, J-N Yue, D-Q Guo

  • 1Department of Vascular Surgery, Zhongshan Hospital, Fudan University, Shanghai, China. fu.weiguo@zs-hospital.sh.cn.

European Review for Medical and Pharmacological Sciences
|August 8, 2019
PubMed
Abstract

Insights

MicroRNA-126 (miR-126) exacerbates lower limb arteriosclerosis obliterans (ASO) by inhibiting the PI3K/Akt pathway, leading to increased endothelial cell apoptosis. Inhibiting miR-126 protects against ASO progression.

Area of Science:

  • Vascular Biology
  • Molecular Medicine
  • Biochemistry

Background:

  • Arteriosclerosis obliterans (ASO) is a significant cause of lower limb ischemia.
  • The role of microRNAs in ASO pathogenesis requires further elucidation.
  • Endothelial dysfunction is a key feature of ASO.

Purpose of the Study:

  • To investigate the role of microRNA-126 (miR-126) in the development of lower limb arteriosclerosis obliterans (ASO) in a rat model.
  • To explore the molecular mechanisms by which miR-126 influences endothelial cell apoptosis and the PI3K/Akt signaling pathway.

Main Methods:

  • ASO model established in male Sprague-Dawley rats.
  • miR-126 expression analyzed using qRT-PCR.
  • PI3K/Akt signaling pathway assessed via Western blotting.
  • Human umbilical vein endothelial cells (HUVECs) treated with oxidized low-density lipoprotein (Ox-LDL) to induce injury.
  • Luciferase assay used to identify miR-126 targets.

Main Results:

  • miR-126 expression was upregulated in ASO rat vessels, correlating with weakened PI3K/Akt signaling.
  • Oxidized low-density lipoprotein (Ox-LDL) induced endothelial cell apoptosis and increased miR-126 expression.
  • PI3K regulatory subunit 2 (PI3KR2) identified as a direct target of miR-126.
  • Inhibition of miR-126 suppressed Ox-LDL-induced apoptosis and activated the PI3K/Akt pathway.

Conclusions:

  • miR-126 plays a detrimental role in lower limb arteriosclerosis obliterans (ASO) by repressing the PI3K/Akt signaling pathway.
  • This repression by miR-126 accelerates endothelial cell apoptosis, contributing to ASO progression.
  • Targeting miR-126 may offer a therapeutic strategy for ASO.

More Related Videos

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells09:58

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

8.0K
Mouse Models for Graft Arteriosclerosis07:37

Mouse Models for Graft Arteriosclerosis

Published on: May 14, 2013

14.1K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.4K
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
14.2K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.7K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

3.8K
Arteries of Lower Limbs01:20

Arteries of Lower Limbs

The external iliac artery transitions out of the body cavity, entering the femoral region of the lower leg, and is renamed the femoral artery at the point where it traverses the body wall. This artery is responsible for the distribution of blood to the thigh's deep muscles and the skin's ventral and lateral regions, achieved through several minor branches and the lateral deep femoral artery, which also spawns a lateral circumflex artery. The knee area receives blood from the genicular...
4.3K
Veins of Lower Limbs01:15

Veins of Lower Limbs

The human body consists of an intricate network of veins responsible for the crucial task of blood drainage from the lower limbs. These veins can be categorized into two main types: deep veins and superficial veins.
Formed by the union of the medial and lateral plantar veins, the posterior tibial vein, rising through the calf muscle, assimilates the fibular vein. The anterior tibial vein, a superior extension of the foot's dorsalis pedis vein, merges with the posterior tibial vein at the...
2.3K